ssr_primer
Primer Details for TRINITY_DN12174_c4_g15_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTGGAGTAAGCTGCTGCA | AACGGGCATACTCAAAGCGA | Start: 283 |
| Tm | 59.892 | 60.037 | End: 508 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
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| Sequence | TCGCTTTGAGTATGCCCGTT | TCTAGAGGGATCAGGAGGCG | Start: 489 |
| Tm | 60.037 | 59.889 | End: 597 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
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| Sequence | TGTTGGAGTAAGCTGCTGCA | ACGGGCATACTCAAAGCGAT | Start: 283 |
| Tm | 59.892 | 59.822 | End: 507 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
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| Sequence | ATCGCTTTGAGTATGCCCGT | TCTAGAGGGATCAGGAGGCG | Start: 488 |
| Tm | 59.822 | 59.889 | End: 597 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
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| Sequence | GTGCAATGCAGTCAAGGCTT | AACGGGCATACTCAAAGCGA | Start: 402 |
| Tm | 59.684 | 60.037 | End: 508 |
| Size | 20 | 20 | 107 |
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