Primer Details for TRINITY_DN12174_c10_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACAAAGAAAGGGAAAAATCGCA | ACAAAGAAGCTTGTCTCCTTTTCA | Start: 9 |
| Tm | 60.142 | 59.05 | End: 159 |
| Size | 24 | 24 | 151 |
| Primer 2 details | |||
| Sequence | CACAAAGAAAGGGAAAAATCGCA | ACAAAGAAGCTTGTCTCCTTTTCA | Start: 10 |
| Tm | 58.633 | 59.05 | End: 159 |
| Size | 23 | 24 | 150 |
| Primer 3 details | |||
| Sequence | ACACAAAGAAAGGGAAAAATCGC | ACAAAGAAGCTTGTCTCCTTTTCA | Start: 9 |
| Tm | 58.632 | 59.05 | End: 159 |
| Size | 23 | 24 | 151 |
| Primer 4 details | |||
| Sequence | ACACAAAGAAAGGGAAAAATCGCA | ACAAAGAAGCTTGTCTCCTTTTCAA | Start: 9 |
| Tm | 60.142 | 59.581 | End: 159 |
| Size | 24 | 25 | 151 |
| Primer 5 details | |||
| Sequence | ACACAAAGAAAGGGAAAAATCGCA | AACAAAGAAGCTTGTCTCCTTTTCA | Start: 9 |
| Tm | 60.142 | 59.581 | End: 160 |
| Size | 24 | 25 | 152 |
