ssr_primer
Primer Details for TRINITY_DN12167_c0_g2_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTATGGACCCCGGATGAA | AAGGCTCGCTACTGCACAAT | Start: 1303 |
| Tm | 60.031 | 60.036 | End: 1502 |
| Size | 20 | 20 | 200 |
| Primer 2 details |
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| Sequence | ACTACCACAGGCAACATGGG | TTCATCCGGGGTCCATAGGT | Start: 1172 |
| Tm | 59.962 | 60.031 | End: 1322 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
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| Sequence | TCATTGGCTCACACCTGGTC | CCAACATGTTCAGTGTGCCG | Start: 547 |
| Tm | 59.963 | 60.04 | End: 816 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
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| Sequence | CGGCACACTGAACATGTTGG | AGCTTTCGTCTTGCGGATGA | Start: 797 |
| Tm | 60.04 | 60.037 | End: 906 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
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| Sequence | ACCTATGGACCCCGGATGAA | TCTTGTCTGTGTTCCGGGTG | Start: 1303 |
| Tm | 60.031 | 59.894 | End: 1534 |
| Size | 20 | 20 | 232 |
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