ssr_primer
Primer Details for TRINITY_DN12163_c0_g8_i2 |
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| (TCT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGAGCTTCGTCTTGGACTT | ATCGCCACTTACTTTGGGGG | Start: 287 |
| Tm | 60.038 | 60.034 | End: 419 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
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| Sequence | GCCTCACTCTGCTCCCATTT | AAGTCCAAGACGAAGCTCGG | Start: 81 |
| Tm | 60.035 | 60.038 | End: 306 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
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| Sequence | CGGCTTTTCTTGTCCCTCCT | AAGTCCAAGACGAAGCTCGG | Start: 35 |
| Tm | 59.963 | 60.038 | End: 306 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
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| Sequence | CCGAGCTTCGTCTTGGACTT | TTTGGGGGAGGTGCAGAATC | Start: 287 |
| Tm | 60.038 | 59.96 | End: 407 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
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| Sequence | CCGAGCTTCGTCTTGGACTT | CTTTGGGGGAGGTGCAGAAT | Start: 287 |
| Tm | 60.038 | 59.96 | End: 408 |
| Size | 20 | 20 | 122 |
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