ssr_primer
Primer Details for TRINITY_DN12161_c0_g5_i3 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTAACTTTCTGCCCAGGGG | TTGGCGTGATAGGTGCTGTT | Start: 55 |
| Tm | 60.034 | 59.964 | End: 330 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | TTACGCCAAGCTACCAGTGG | AGGAAGAAGAGGCCGTGTTG | Start: 821 |
| Tm | 60.037 | 59.965 | End: 994 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | TGCAACCCAGACGGCTAATT | AGGAAGAAGAGGCCGTGTTG | Start: 727 |
| Tm | 59.962 | 59.965 | End: 994 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | TTGCAACCCAGACGGCTAAT | AGGAAGAAGAGGCCGTGTTG | Start: 726 |
| Tm | 59.962 | 59.965 | End: 994 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | TGGCGTTACTGAATTCGGCT | CCACTGGTAGCTTGGCGTAA | Start: 612 |
| Tm | 60.037 | 60.037 | End: 840 |
| Size | 20 | 20 | 229 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTAACTTTCTGCCCAGGGG | TTGGCGTGATAGGTGCTGTT | Start: 55 |
| Tm | 60.034 | 59.964 | End: 330 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | TTACGCCAAGCTACCAGTGG | AGGAAGAAGAGGCCGTGTTG | Start: 821 |
| Tm | 60.037 | 59.965 | End: 994 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | TGCAACCCAGACGGCTAATT | AGGAAGAAGAGGCCGTGTTG | Start: 727 |
| Tm | 59.962 | 59.965 | End: 994 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | TTGCAACCCAGACGGCTAAT | AGGAAGAAGAGGCCGTGTTG | Start: 726 |
| Tm | 59.962 | 59.965 | End: 994 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | TGGCGTTACTGAATTCGGCT | CCACTGGTAGCTTGGCGTAA | Start: 612 |
| Tm | 60.037 | 60.037 | End: 840 |
| Size | 20 | 20 | 229 |
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