ssr_primer
Primer Details for TRINITY_DN12160_c3_g3_i1 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAAACCCTTTCTCCCCGA | GATAGGTGGCATGGACGGAG | Start: 434 |
| Tm | 59.964 | 59.966 | End: 664 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | CCAAGGGGGTGAATCAAGCT | GCCCGAACACCTCAAAGAGA | Start: 1232 |
| Tm | 59.96 | 59.965 | End: 1483 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TGTTGCCAGGCGAAGAAGAT | ATTATGCTGGTTGGGGCCAA | Start: 3357 |
| Tm | 59.963 | 59.958 | End: 3501 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
|---|
| Sequence | GTTGTTGCCAGGCGAAGAAG | ATTATGCTGGTTGGGGCCAA | Start: 3355 |
| Tm | 60.04 | 59.958 | End: 3501 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | ACGGCCGACAAAAACCAAAG | GATAGGTGGCATGGACGGAG | Start: 518 |
| Tm | 59.898 | 59.966 | End: 664 |
| Size | 20 | 20 | 147 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAAACCCTTTCTCCCCGA | GATAGGTGGCATGGACGGAG | Start: 434 |
| Tm | 59.964 | 59.966 | End: 664 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | CCAAGGGGGTGAATCAAGCT | GCCCGAACACCTCAAAGAGA | Start: 1232 |
| Tm | 59.96 | 59.965 | End: 1483 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TGTTGCCAGGCGAAGAAGAT | ATTATGCTGGTTGGGGCCAA | Start: 3357 |
| Tm | 59.963 | 59.958 | End: 3501 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
|---|
| Sequence | GTTGTTGCCAGGCGAAGAAG | ATTATGCTGGTTGGGGCCAA | Start: 3355 |
| Tm | 60.04 | 59.958 | End: 3501 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | ACGGCCGACAAAAACCAAAG | GATAGGTGGCATGGACGGAG | Start: 518 |
| Tm | 59.898 | 59.966 | End: 664 |
| Size | 20 | 20 | 147 |
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