ssr_primer
Primer Details for TRINITY_DN12147_c1_g2_i11 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGAACACGGCGTCTCCAAT | AGCGTCTCTGCATTGTCACA | Start: 3520 |
| Tm | 60.036 | 59.966 | End: 3722 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TGTGACAATGCAGAGACGCT | AGTCGGACATGGACTGCTTG | Start: 3703 |
| Tm | 59.966 | 60.037 | End: 3971 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
|---|
| Sequence | TGTGACAATGCAGAGACGCT | TTGAGTCGGACATGGACTGC | Start: 3703 |
| Tm | 59.966 | 60.037 | End: 3974 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | CCACCTTCACGATCCAAGCT | TCAGCAGCCTCTTCAAGTGG | Start: 4490 |
| Tm | 60.036 | 59.964 | End: 4682 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | TCCACCTTCACGATCCAAGC | TCAGCAGCCTCTTCAAGTGG | Start: 4489 |
| Tm | 60.036 | 59.964 | End: 4682 |
| Size | 20 | 20 | 194 |
(A)11gaacatttaagtttcacaattctaaactctaaaaaaattaatgtggtgtttctttcatcatctttgtacaacttttgacaagtggtcagg(A)10ctcatttacaaccaaatgaaaaggcaagtgggttacacttgaacatccctaatcaaacaccactaa(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGAACACGGCGTCTCCAAT | AGCGTCTCTGCATTGTCACA | Start: 3520 |
| Tm | 60.036 | 59.966 | End: 3722 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TGTGACAATGCAGAGACGCT | AGTCGGACATGGACTGCTTG | Start: 3703 |
| Tm | 59.966 | 60.037 | End: 3971 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
|---|
| Sequence | TGTGACAATGCAGAGACGCT | TTGAGTCGGACATGGACTGC | Start: 3703 |
| Tm | 59.966 | 60.037 | End: 3974 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | CCACCTTCACGATCCAAGCT | TCAGCAGCCTCTTCAAGTGG | Start: 4490 |
| Tm | 60.036 | 59.964 | End: 4682 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | TCCACCTTCACGATCCAAGC | TCAGCAGCCTCTTCAAGTGG | Start: 4489 |
| Tm | 60.036 | 59.964 | End: 4682 |
| Size | 20 | 20 | 194 |
(A)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGAACACGGCGTCTCCAAT | AGCGTCTCTGCATTGTCACA | Start: 3520 |
| Tm | 60.036 | 59.966 | End: 3722 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TGTGACAATGCAGAGACGCT | AGTCGGACATGGACTGCTTG | Start: 3703 |
| Tm | 59.966 | 60.037 | End: 3971 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
|---|
| Sequence | TGTGACAATGCAGAGACGCT | TTGAGTCGGACATGGACTGC | Start: 3703 |
| Tm | 59.966 | 60.037 | End: 3974 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | CCACCTTCACGATCCAAGCT | TCAGCAGCCTCTTCAAGTGG | Start: 4490 |
| Tm | 60.036 | 59.964 | End: 4682 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | TCCACCTTCACGATCCAAGC | TCAGCAGCCTCTTCAAGTGG | Start: 4489 |
| Tm | 60.036 | 59.964 | End: 4682 |
| Size | 20 | 20 | 194 |
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