ssr_primer
Primer Details for TRINITY_DN12141_c7_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGGACGCTCCTCCCCTTA | AGGGACACGTCAAAACCACA | Start: 1040 |
| Tm | 60.546 | 59.746 | End: 1210 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
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| Sequence | TACTTTTGGACGCTCCTCCC | AGGGACACGTCAAAACCACA | Start: 1036 |
| Tm | 59.388 | 59.746 | End: 1210 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
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| Sequence | TTGGGTTTCCCTTTTGGCCA | TAAGGGGAGGAGCGTCCAAA | Start: 802 |
| Tm | 60.327 | 60.546 | End: 1059 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
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| Sequence | TTGGGTTTCCCTTTTGGCCA | GGGAGGAGCGTCCAAAAGTA | Start: 802 |
| Tm | 60.327 | 59.388 | End: 1055 |
| Size | 20 | 20 | 254 |
| Primer 5 details |
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| Sequence | CCTCCCCTTACGTTCCATGT | AGGGACACGTCAAAACCACA | Start: 1050 |
| Tm | 59.09 | 59.746 | End: 1210 |
| Size | 20 | 20 | 161 |
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