ssr_primer
Primer Details for TRINITY_DN12135_c1_g8_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCATTGGTTGCTGGAGCCT | TCCGGTAGGCTTTTCGCAAT | Start: 1113 |
| Tm | 59.887 | 60.036 | End: 1360 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
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| Sequence | TTCATTGGTTGCTGGAGCCT | CCGGTAGGCTTTTCGCAATG | Start: 1113 |
| Tm | 59.887 | 59.901 | End: 1359 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
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| Sequence | TCGGGGCTCTAAGTGGAAGA | CCCGCATTAGGCACTAGCTT | Start: 1478 |
| Tm | 59.959 | 60.179 | End: 1610 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
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| Sequence | CTCGCAAGCAGATGCAAGTC | CCCGCATTAGGCACTAGCTT | Start: 1460 |
| Tm | 59.903 | 60.179 | End: 1610 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
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| Sequence | CAAGTCGGGGCTCTAAGTGG | CCCGCATTAGGCACTAGCTT | Start: 1474 |
| Tm | 60.108 | 60.179 | End: 1610 |
| Size | 20 | 20 | 137 |
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