ssr_primer
Primer Details for TRINITY_DN12135_c1_g6_i4 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGAAGTTCCTCAATGGCA | AATGCAACAACCCTTCCCCA | Start: 619 |
| Tm | 59.96 | 60.105 | End: 718 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | GGGGAAGTTCCTCAATGGCA | TCCTCCAACCCCAACCATTG | Start: 619 |
| Tm | 59.96 | 59.886 | End: 886 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | TTTCAACCAACCGGCTAGCT | TGCAACAACCCTTCCCCATT | Start: 591 |
| Tm | 59.89 | 60.105 | End: 716 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TTTCAACCAACCGGCTAGCT | AATGCAACAACCCTTCCCCA | Start: 591 |
| Tm | 59.89 | 60.105 | End: 718 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | GGGGAAGTTCCTCAATGGCA | CCTCCAACCCCAACCATTGT | Start: 619 |
| Tm | 59.96 | 60.179 | End: 885 |
| Size | 20 | 20 | 267 |
(AG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGAAGTTCCTCAATGGCA | AATGCAACAACCCTTCCCCA | Start: 619 |
| Tm | 59.96 | 60.105 | End: 718 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | GGGGAAGTTCCTCAATGGCA | TCCTCCAACCCCAACCATTG | Start: 619 |
| Tm | 59.96 | 59.886 | End: 886 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | TTTCAACCAACCGGCTAGCT | TGCAACAACCCTTCCCCATT | Start: 591 |
| Tm | 59.89 | 60.105 | End: 716 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TTTCAACCAACCGGCTAGCT | AATGCAACAACCCTTCCCCA | Start: 591 |
| Tm | 59.89 | 60.105 | End: 718 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | GGGGAAGTTCCTCAATGGCA | CCTCCAACCCCAACCATTGT | Start: 619 |
| Tm | 59.96 | 60.179 | End: 885 |
| Size | 20 | 20 | 267 |
(T)12agtttatttcttcaaatgttcttggaa(AG)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGAAGTTCCTCAATGGCA | AATGCAACAACCCTTCCCCA | Start: 619 |
| Tm | 59.96 | 60.105 | End: 718 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | GGGGAAGTTCCTCAATGGCA | TCCTCCAACCCCAACCATTG | Start: 619 |
| Tm | 59.96 | 59.886 | End: 886 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | TTTCAACCAACCGGCTAGCT | TGCAACAACCCTTCCCCATT | Start: 591 |
| Tm | 59.89 | 60.105 | End: 716 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TTTCAACCAACCGGCTAGCT | AATGCAACAACCCTTCCCCA | Start: 591 |
| Tm | 59.89 | 60.105 | End: 718 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | GGGGAAGTTCCTCAATGGCA | CCTCCAACCCCAACCATTGT | Start: 619 |
| Tm | 59.96 | 60.179 | End: 885 |
| Size | 20 | 20 | 267 |
ICAR Data Use Licence
