ssr_primer
Primer Details for TRINITY_DN12131_c2_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGGACGACTAACCAATTCCT | TCTCGGTTATTGGCTTCGGG | Start: 39 |
| Tm | 59.096 | 59.823 | End: 232 |
| Size | 22 | 20 | 194 |
| Primer 2 details |
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| Sequence | ACTGGACGACTAACCAATTCCT | CGGTTATTGGCTTCGGGCTA | Start: 39 |
| Tm | 59.096 | 60.179 | End: 229 |
| Size | 22 | 20 | 191 |
| Primer 3 details |
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| Sequence | ACTGGACGACTAACCAATTCCT | CAGGAAGCGGATTCTCTCGG | Start: 39 |
| Tm | 59.096 | 60.249 | End: 246 |
| Size | 22 | 20 | 208 |
| Primer 4 details |
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| Sequence | TGGGACTATTAGAATCCGCTTCC | TCTCGGTTATTGGCTTCGGG | Start: 133 |
| Tm | 59.676 | 59.823 | End: 232 |
| Size | 23 | 20 | 100 |
| Primer 5 details |
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| Sequence | ACTGGACGACTAACCAATTCCTT | TCTCGGTTATTGGCTTCGGG | Start: 39 |
| Tm | 59.673 | 59.823 | End: 232 |
| Size | 23 | 20 | 194 |
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