ssr_primer
Primer Details for TRINITY_DN12122_c0_g2_i5 |
|---|
| (TG)9: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCCAAACCATCCGGATC | GACGCGCTTTTGTTGAAGGT | Start: 5225 |
| Tm | 60.034 | 59.971 | End: 5397 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | ACACCCAAACCATCCGGATC | GAGAGATGGGTGGGGAGACT | Start: 5225 |
| Tm | 60.034 | 60.032 | End: 5356 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CGACGCACAAACCATGTTGT | GTTGCGGTTGCTATGCCAAA | Start: 4192 |
| Tm | 59.971 | 60.039 | End: 4428 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
|---|
| Sequence | ACGACGCACAAACCATGTTG | GTTGCGGTTGCTATGCCAAA | Start: 4191 |
| Tm | 59.971 | 60.039 | End: 4428 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | CCGCTCTTCTTGATGCTTGC | GATCCGGATGGTTTGGGTGT | Start: 5066 |
| Tm | 59.902 | 60.034 | End: 5244 |
| Size | 20 | 20 | 179 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCCAAACCATCCGGATC | GACGCGCTTTTGTTGAAGGT | Start: 5225 |
| Tm | 60.034 | 59.971 | End: 5397 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | ACACCCAAACCATCCGGATC | GAGAGATGGGTGGGGAGACT | Start: 5225 |
| Tm | 60.034 | 60.032 | End: 5356 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CGACGCACAAACCATGTTGT | GTTGCGGTTGCTATGCCAAA | Start: 4192 |
| Tm | 59.971 | 60.039 | End: 4428 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
|---|
| Sequence | ACGACGCACAAACCATGTTG | GTTGCGGTTGCTATGCCAAA | Start: 4191 |
| Tm | 59.971 | 60.039 | End: 4428 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | CCGCTCTTCTTGATGCTTGC | GATCCGGATGGTTTGGGTGT | Start: 5066 |
| Tm | 59.902 | 60.034 | End: 5244 |
| Size | 20 | 20 | 179 |
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