Primer Details for TRINITY_DN12115_c4_g1_i1 |
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| (TA)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAAAATTCATTTCCTTGTCTTCA | GGGTTTAATGGGCTCTGATACCA | Start: 225 |
| Tm | 57.962 | 60.118 | End: 426 |
| Size | 25 | 23 | 202 |
| Primer 2 details |
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| Sequence | ACGAAAATTCATTTCCTTGTCTTCA | TGGGTTTAATGGGCTCTGATACC | Start: 225 |
| Tm | 57.962 | 60.118 | End: 427 |
| Size | 25 | 23 | 203 |
| Primer 3 details |
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| Sequence | TGAAGTCACATTTGAATGTTTGAGT | GGGTTTAATGGGCTCTGATACCA | Start: 262 |
| Tm | 57.901 | 60.118 | End: 426 |
| Size | 25 | 23 | 165 |
| Primer 4 details |
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| Sequence | TGAAGTCACATTTGAATGTTTGAGT | TGGGTTTAATGGGCTCTGATACC | Start: 262 |
| Tm | 57.901 | 60.118 | End: 427 |
| Size | 25 | 23 | 166 |
| Primer 5 details |
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| Sequence | TCTTCGGAAGCTTTTGAAGGT | TGAAGACAAGGAAATGAATTTTCGT | Start: 44 |
| Tm | 57.791 | 57.962 | End: 249 |
| Size | 21 | 25 | 206 |
