Primer Details for TRINITY_DN12115_c3_g1_i1 |
|---|
| (AAT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCCTGTTTTTCTTCCTCCA | TGGAATTAGGGAATTAGGTTTTGGT | Start: 97 |
| Tm | 59.858 | 58.433 | End: 259 |
| Size | 21 | 25 | 163 |
| Primer 2 details |
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| Sequence | CGCCTGTTTTTCTTCCTCCA | TGGAATTAGGGAATTAGGTTTTGGT | Start: 98 |
| Tm | 58.395 | 58.433 | End: 259 |
| Size | 20 | 25 | 162 |
| Primer 3 details |
|---|
| Sequence | TCGCCTGTTTTTCTTCCTCCA | ATGGAATTAGGGAATTAGGTTTTGGT | Start: 97 |
| Tm | 59.858 | 58.804 | End: 260 |
| Size | 21 | 26 | 164 |
| Primer 4 details |
|---|
| Sequence | TCGCCTGTTTTTCTTCCTCC | TGGAATTAGGGAATTAGGTTTTGGT | Start: 97 |
| Tm | 58.108 | 58.433 | End: 259 |
| Size | 20 | 25 | 163 |
| Primer 5 details |
|---|
| Sequence | TCGCCTGTTTTTCTTCCTCCA | GATGGAATTAGGGAATTAGGTTTTGGT | Start: 97 |
| Tm | 59.858 | 59.655 | End: 261 |
| Size | 21 | 27 | 165 |
