Primer Details for TRINITY_DN12105_c3_g1_i1 | |||
|---|---|---|---|
| (AT)7: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCCATTTGAAACCCTAATGAC | TCGCATTATGTTGATAACGAAAGA | Start: 3 |
| Tm | 59.486 | 57.104 | End: 224 |
| Size | 23 | 24 | 222 |
| Primer 2 details | |||
| Sequence | GGCCATTTGAAACCCTAATGACT | TCGCATTATGTTGATAACGAAAGA | Start: 4 |
| Tm | 59.231 | 57.104 | End: 224 |
| Size | 23 | 24 | 221 |
| Primer 3 details | |||
| Sequence | TGGCCATTTGAAACCCTAATGA | TCGCATTATGTTGATAACGAAAGA | Start: 3 |
| Tm | 58.216 | 57.104 | End: 224 |
| Size | 22 | 24 | 222 |
| Primer 4 details | |||
| Sequence | TGGCCATTTGAAACCCTAATGAC | TCGCATTATGTTGATAACGAAAGAA | Start: 3 |
| Tm | 59.486 | 57.703 | End: 224 |
| Size | 23 | 25 | 222 |
| Primer 5 details | |||
| Sequence | TGGCCATTTGAAACCCTAATGAC | TTGTAATTGTACGTAGTCTAGGGTT | Start: 3 |
| Tm | 59.486 | 57.533 | End: 142 |
| Size | 23 | 25 | 140 |
