ssr_primer
Primer Details for TRINITY_DN12100_c1_g4_i4 |
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| (T)11aaaaaaaattaatccgttcttttcttgagg(CT)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCCCACGCAATCGAGAT | TTCGTTCACCTGGGGCATAC | Start: 1628 |
| Tm | 60.035 | 60.036 | End: 1842 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
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| Sequence | CAATCAGCTGGACACGGACT | TTCGTTCACCTGGGGCATAC | Start: 1701 |
| Tm | 60.037 | 60.036 | End: 1842 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
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| Sequence | AAACCCCACGCAATCGAGAT | TGCCCCACAATCACCTTCTC | Start: 1628 |
| Tm | 60.035 | 59.962 | End: 1763 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
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| Sequence | AAACCCCACGCAATCGAGAT | TCCTAGGAGATTGCCCCACA | Start: 1628 |
| Tm | 60.035 | 59.956 | End: 1774 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | GAGGCCCAGTGTGAATCTCC | ATCTCGATTGCGTGGGGTTT | Start: 1507 |
| Tm | 60.107 | 60.035 | End: 1647 |
| Size | 20 | 20 | 141 |
(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCCCACGCAATCGAGAT | TTCGTTCACCTGGGGCATAC | Start: 1628 |
| Tm | 60.035 | 60.036 | End: 1842 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | CAATCAGCTGGACACGGACT | TTCGTTCACCTGGGGCATAC | Start: 1701 |
| Tm | 60.037 | 60.036 | End: 1842 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | AAACCCCACGCAATCGAGAT | TGCCCCACAATCACCTTCTC | Start: 1628 |
| Tm | 60.035 | 59.962 | End: 1763 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | AAACCCCACGCAATCGAGAT | TCCTAGGAGATTGCCCCACA | Start: 1628 |
| Tm | 60.035 | 59.956 | End: 1774 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GAGGCCCAGTGTGAATCTCC | ATCTCGATTGCGTGGGGTTT | Start: 1507 |
| Tm | 60.107 | 60.035 | End: 1647 |
| Size | 20 | 20 | 141 |
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