ssr_primer
Primer Details for TRINITY_DN12091_c0_g5_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCCCGATGAAGTTGAGG | CGTCCTTTGCTGCCTACTGA | Start: 432 |
| Tm | 60.107 | 60.037 | End: 709 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | GCATCGGGTTGGTTTTCTGG | CCTCAACTTCATCGGGCCAT | Start: 321 |
| Tm | 59.756 | 60.107 | End: 451 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | GAGCAGCGACAGAAGATGGT | CCAGAAAACCAACCCGATGC | Start: 105 |
| Tm | 60.108 | 59.756 | End: 340 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | TGAGCAGCGACAGAAGATGG | CCAGAAAACCAACCCGATGC | Start: 104 |
| Tm | 60.108 | 59.756 | End: 340 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | GGTTGGTTTTCTGGTGCTGC | CCTCAACTTCATCGGGCCAT | Start: 327 |
| Tm | 60.249 | 60.107 | End: 451 |
| Size | 20 | 20 | 125 |
(A)10tatactataatcaagcttggaaaact(ACA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCCCGATGAAGTTGAGG | CGTCCTTTGCTGCCTACTGA | Start: 432 |
| Tm | 60.107 | 60.037 | End: 709 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | GCATCGGGTTGGTTTTCTGG | CCTCAACTTCATCGGGCCAT | Start: 321 |
| Tm | 59.756 | 60.107 | End: 451 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | GAGCAGCGACAGAAGATGGT | CCAGAAAACCAACCCGATGC | Start: 105 |
| Tm | 60.108 | 59.756 | End: 340 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | TGAGCAGCGACAGAAGATGG | CCAGAAAACCAACCCGATGC | Start: 104 |
| Tm | 60.108 | 59.756 | End: 340 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | GGTTGGTTTTCTGGTGCTGC | CCTCAACTTCATCGGGCCAT | Start: 327 |
| Tm | 60.249 | 60.107 | End: 451 |
| Size | 20 | 20 | 125 |
(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCCCGATGAAGTTGAGG | CGTCCTTTGCTGCCTACTGA | Start: 432 |
| Tm | 60.107 | 60.037 | End: 709 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | GCATCGGGTTGGTTTTCTGG | CCTCAACTTCATCGGGCCAT | Start: 321 |
| Tm | 59.756 | 60.107 | End: 451 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | GAGCAGCGACAGAAGATGGT | CCAGAAAACCAACCCGATGC | Start: 105 |
| Tm | 60.108 | 59.756 | End: 340 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | TGAGCAGCGACAGAAGATGG | CCAGAAAACCAACCCGATGC | Start: 104 |
| Tm | 60.108 | 59.756 | End: 340 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | GGTTGGTTTTCTGGTGCTGC | CCTCAACTTCATCGGGCCAT | Start: 327 |
| Tm | 60.249 | 60.107 | End: 451 |
| Size | 20 | 20 | 125 |
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