ssr_primer
Primer Details for TRINITY_DN12087_c0_g1_i13 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCGTTGCTGTGGACTTC | ATAAGAGGGTCGGTCAGGCT | Start: 4735 |
| Tm | 59.966 | 60.032 | End: 4844 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
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| Sequence | GCTCTCAAACGGTCAGACCA | CAAGCCCAGTCCCCAGTTAG | Start: 373 |
| Tm | 59.966 | 60.035 | End: 584 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
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| Sequence | ATGGGCTCTCAAACGGTCAG | CAAGCCCAGTCCCCAGTTAG | Start: 369 |
| Tm | 60.036 | 60.035 | End: 584 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
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| Sequence | ATTCACGTTGCGCATTGCTT | GAAGTCCACAGCAACGGAGA | Start: 4508 |
| Tm | 60.039 | 59.966 | End: 4754 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
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| Sequence | CGTTGGAATTACTGGCGCTG | CAAGCCCAGTCCCCAGTTAG | Start: 400 |
| Tm | 59.903 | 60.035 | End: 584 |
| Size | 20 | 20 | 185 |
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