Primer Details for TRINITY_DN12083_c5_g1_i1 |
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| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCCCTATTTTCATGCATTATGC | TCATTTTGGATGTGGTGTATTTACTT | Start: 48 |
| Tm | 59.755 | 57.584 | End: 188 |
| Size | 25 | 26 | 141 |
| Primer 2 details |
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| Sequence | ACTCCCCTATTTTCATGCATTATGC | CCTTAATTATCATTTTGGATGTGGTGT | Start: 48 |
| Tm | 59.755 | 58.523 | End: 197 |
| Size | 25 | 27 | 150 |
| Primer 3 details |
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| Sequence | CCCCTATTTTCATGCATTATGCTCC | TCATTTTGGATGTGGTGTATTTACTT | Start: 51 |
| Tm | 60.337 | 57.584 | End: 188 |
| Size | 25 | 26 | 138 |
| Primer 4 details |
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| Sequence | TCCCCTATTTTCATGCATTATGCT | TCATTTTGGATGTGGTGTATTTACTT | Start: 50 |
| Tm | 58.604 | 57.584 | End: 188 |
| Size | 24 | 26 | 139 |
| Primer 5 details |
|---|
| Sequence | CCCCTATTTTCATGCATTATGCTCC | CCTTAATTATCATTTTGGATGTGGTGT | Start: 51 |
| Tm | 60.337 | 58.523 | End: 197 |
| Size | 25 | 27 | 147 |
