ssr_primer
Primer Details for TRINITY_DN12069_c0_g2_i2 |
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| (T)10gtttttttcgcggttcaatcagtcgaggtaggtatatgattttttcattgtcttttaa(T)10gtcatgagttttggttaatctgaatccacagagattaatatgaatgcatggataattggatttgatcacttgtgcgtccgtttgacgagaatttttca(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTTCACGCAAAAGACGCC | TGACTTCCTGCGTTCTTCCC | Start: 1654 |
| Tm | 59.971 | 59.965 | End: 1918 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
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| Sequence | ATCAGGGTCCCAGGCAAATG | ATCTGTCTCGCGCTTATGGG | Start: 2131 |
| Tm | 60.033 | 59.967 | End: 2335 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
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| Sequence | GGGAAGAACGCAGGAAGTCA | CATTTGCCTGGGACCCTGAT | Start: 1899 |
| Tm | 59.965 | 60.033 | End: 2150 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
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| Sequence | CTGAAATGCCCCATAAGCGC | GAACTGCCCCACTTCCATCA | Start: 2307 |
| Tm | 59.969 | 59.962 | End: 2506 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
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| Sequence | CTGAAATGCCCCATAAGCGC | TGAACTGCCCCACTTCCATC | Start: 2307 |
| Tm | 59.969 | 59.962 | End: 2507 |
| Size | 20 | 20 | 201 |
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