ssr_primer
Primer Details for TRINITY_DN12063_c2_g5_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGAGAACCCGTTCGTTT | CGCGAGCTTCGAAGAAATGG | Start: 2316 |
| Tm | 59.966 | 59.974 | End: 2431 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | TGAATAGCGCCGTATCCCAC | GCCGTAGAGGAGGTATGCAC | Start: 1592 |
| Tm | 59.967 | 59.968 | End: 1809 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | AATAGCGCCGTATCCCACTG | GCCGTAGAGGAGGTATGCAC | Start: 1594 |
| Tm | 59.966 | 59.968 | End: 1809 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | GCTCCTTTTGCTTTTCGGCA | GAGGTTGCCTTGGTGCCTAT | Start: 1291 |
| Tm | 59.968 | 60.034 | End: 1407 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | CGCCCTTCACACCCTACAAT | GTGGGATACGGCGCTATTCA | Start: 1363 |
| Tm | 60.035 | 59.967 | End: 1611 |
| Size | 20 | 20 | 249 |
(T)21: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGAGAACCCGTTCGTTT | CGCGAGCTTCGAAGAAATGG | Start: 2316 |
| Tm | 59.966 | 59.974 | End: 2431 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | TGAATAGCGCCGTATCCCAC | GCCGTAGAGGAGGTATGCAC | Start: 1592 |
| Tm | 59.967 | 59.968 | End: 1809 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | AATAGCGCCGTATCCCACTG | GCCGTAGAGGAGGTATGCAC | Start: 1594 |
| Tm | 59.966 | 59.968 | End: 1809 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | GCTCCTTTTGCTTTTCGGCA | GAGGTTGCCTTGGTGCCTAT | Start: 1291 |
| Tm | 59.968 | 60.034 | End: 1407 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | CGCCCTTCACACCCTACAAT | GTGGGATACGGCGCTATTCA | Start: 1363 |
| Tm | 60.035 | 59.967 | End: 1611 |
| Size | 20 | 20 | 249 |
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