ssr_primer
Primer Details for TRINITY_DN12060_c2_g1_i2 |
|---|
| (CTT)5caccacctccaccaccac(CAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTCTCTCCGATCCTTCA | GGTAGCTTTCCCTTGAGCGT | Start: 640 |
| Tm | 60.033 | 60.036 | End: 903 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | AATCAGGGCAGCTGAGTTCC | TGCGCAGGAACTTCAGTGAT | Start: 7130 |
| Tm | 60.035 | 59.965 | End: 7366 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | ATCACTGAAGTTCCTGCGCA | TCGGTCTTTTGGGATCAGGC | Start: 7347 |
| Tm | 59.965 | 60.035 | End: 7479 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | TGCATCCTCTTTGGTGACCC | TTGAGCTTCATGGAGGCTGG | Start: 6606 |
| Tm | 59.962 | 60.035 | End: 6796 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | CAAACCGCGTTGTCCGATTT | CTGGGGTGCATTGTTGTGTG | Start: 2351 |
| Tm | 60.041 | 59.968 | End: 2540 |
| Size | 20 | 20 | 190 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTCTCTCCGATCCTTCA | GGTAGCTTTCCCTTGAGCGT | Start: 640 |
| Tm | 60.033 | 60.036 | End: 903 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | AATCAGGGCAGCTGAGTTCC | TGCGCAGGAACTTCAGTGAT | Start: 7130 |
| Tm | 60.035 | 59.965 | End: 7366 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | ATCACTGAAGTTCCTGCGCA | TCGGTCTTTTGGGATCAGGC | Start: 7347 |
| Tm | 59.965 | 60.035 | End: 7479 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | TGCATCCTCTTTGGTGACCC | TTGAGCTTCATGGAGGCTGG | Start: 6606 |
| Tm | 59.962 | 60.035 | End: 6796 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | CAAACCGCGTTGTCCGATTT | CTGGGGTGCATTGTTGTGTG | Start: 2351 |
| Tm | 60.041 | 59.968 | End: 2540 |
| Size | 20 | 20 | 190 |
(T)10(A)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTCTCTCCGATCCTTCA | GGTAGCTTTCCCTTGAGCGT | Start: 640 |
| Tm | 60.033 | 60.036 | End: 903 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | AATCAGGGCAGCTGAGTTCC | TGCGCAGGAACTTCAGTGAT | Start: 7130 |
| Tm | 60.035 | 59.965 | End: 7366 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | ATCACTGAAGTTCCTGCGCA | TCGGTCTTTTGGGATCAGGC | Start: 7347 |
| Tm | 59.965 | 60.035 | End: 7479 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | TGCATCCTCTTTGGTGACCC | TTGAGCTTCATGGAGGCTGG | Start: 6606 |
| Tm | 59.962 | 60.035 | End: 6796 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | CAAACCGCGTTGTCCGATTT | CTGGGGTGCATTGTTGTGTG | Start: 2351 |
| Tm | 60.041 | 59.968 | End: 2540 |
| Size | 20 | 20 | 190 |
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