ssr_primer
Primer Details for TRINITY_DN12058_c1_g4_i16 |
|---|
| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTAAAGGGGTTGCCATCC | TACGACGGAATGATGGAGCG | Start: 474 |
| Tm | 60.035 | 59.97 | End: 583 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TAGCAACTTCCTGAGCACCG | CTGCCGATCTCTTCGTCCTC | Start: 3987 |
| Tm | 60.037 | 59.97 | End: 4196 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CGCTCCATCATTCCGTCGTA | GGACAGGTTCCGGTCAGAAG | Start: 564 |
| Tm | 59.97 | 60.037 | End: 784 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | TCGTAAAGGGGTTGCCATCC | CACTCGAAGTGTCACCAGCT | Start: 474 |
| Tm | 60.035 | 59.967 | End: 624 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | TAAAGGGGTTGCCATCCCAC | TACGACGGAATGATGGAGCG | Start: 477 |
| Tm | 59.96 | 59.97 | End: 583 |
| Size | 20 | 20 | 107 |
(TC)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTAAAGGGGTTGCCATCC | TACGACGGAATGATGGAGCG | Start: 474 |
| Tm | 60.035 | 59.97 | End: 583 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TAGCAACTTCCTGAGCACCG | CTGCCGATCTCTTCGTCCTC | Start: 3987 |
| Tm | 60.037 | 59.97 | End: 4196 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CGCTCCATCATTCCGTCGTA | GGACAGGTTCCGGTCAGAAG | Start: 564 |
| Tm | 59.97 | 60.037 | End: 784 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | TCGTAAAGGGGTTGCCATCC | CACTCGAAGTGTCACCAGCT | Start: 474 |
| Tm | 60.035 | 59.967 | End: 624 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | TAAAGGGGTTGCCATCCCAC | TACGACGGAATGATGGAGCG | Start: 477 |
| Tm | 59.96 | 59.97 | End: 583 |
| Size | 20 | 20 | 107 |
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