ssr_primer
Primer Details for TRINITY_DN12040_c1_g4_i1 |
|---|
| (TTCT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTCTCCAGTCATACCTCGGA | TTGGAGAGGCTCTAGAGACCC | Start: 103 |
| Tm | 60.025 | 60.064 | End: 202 |
| Size | 22 | 21 | 100 |
| Primer 2 details |
|---|
| Sequence | GATAGGAGGTCCTTGTGCCA | GCTGGTTTGAGCATAGGTAGC | Start: 225 |
| Tm | 58.796 | 59.057 | End: 412 |
| Size | 20 | 21 | 188 |
| Primer 3 details |
|---|
| Sequence | TGATAGGAGGTCCTTGTGCC | GCTGGTTTGAGCATAGGTAGC | Start: 224 |
| Tm | 58.796 | 59.057 | End: 412 |
| Size | 20 | 21 | 189 |
| Primer 4 details |
|---|
| Sequence | GGGACTTGAGGCACCAATCA | ACAACATTACTGCTAGAGACCCC | Start: 128 |
| Tm | 59.962 | 59.802 | End: 304 |
| Size | 20 | 23 | 177 |
| Primer 5 details |
|---|
| Sequence | GATAGGAGGTCCTTGTGCCA | GCCACTTCTGAACATTGTTGCT | Start: 225 |
| Tm | 58.796 | 59.965 | End: 493 |
| Size | 20 | 22 | 269 |
(TA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTCTCCAGTCATACCTCGGA | TTGGAGAGGCTCTAGAGACCC | Start: 103 |
| Tm | 60.025 | 60.064 | End: 202 |
| Size | 22 | 21 | 100 |
| Primer 2 details |
|---|
| Sequence | GATAGGAGGTCCTTGTGCCA | GCTGGTTTGAGCATAGGTAGC | Start: 225 |
| Tm | 58.796 | 59.057 | End: 412 |
| Size | 20 | 21 | 188 |
| Primer 3 details |
|---|
| Sequence | TGATAGGAGGTCCTTGTGCC | GCTGGTTTGAGCATAGGTAGC | Start: 224 |
| Tm | 58.796 | 59.057 | End: 412 |
| Size | 20 | 21 | 189 |
| Primer 4 details |
|---|
| Sequence | GGGACTTGAGGCACCAATCA | ACAACATTACTGCTAGAGACCCC | Start: 128 |
| Tm | 59.962 | 59.802 | End: 304 |
| Size | 20 | 23 | 177 |
| Primer 5 details |
|---|
| Sequence | GATAGGAGGTCCTTGTGCCA | GCCACTTCTGAACATTGTTGCT | Start: 225 |
| Tm | 58.796 | 59.965 | End: 493 |
| Size | 20 | 22 | 269 |
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