Primer Details for TRINITY_DN12034_c2_g8_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTCAATGAGAGAATGCCCA | AGGTAGCAAAATACACCAAAGGT | Start: 47 |
| Tm | 59.082 | 58.264 | End: 210 |
| Size | 21 | 23 | 164 |
| Primer 2 details | |||
| Sequence | GGTCAATGAGAGAATGCCCAC | AGGTAGCAAAATACACCAAAGGT | Start: 48 |
| Tm | 58.98 | 58.264 | End: 210 |
| Size | 21 | 23 | 163 |
| Primer 3 details | |||
| Sequence | AGGTCAATGAGAGAATGCCCA | AGCAAAATACACCAAAGGTAGGA | Start: 47 |
| Tm | 59.082 | 58.007 | End: 206 |
| Size | 21 | 23 | 160 |
| Primer 4 details | |||
| Sequence | GGTCAATGAGAGAATGCCCAC | AGCAAAATACACCAAAGGTAGGA | Start: 48 |
| Tm | 58.98 | 58.007 | End: 206 |
| Size | 21 | 23 | 159 |
| Primer 5 details | |||
| Sequence | AGGTCAATGAGAGAATGCCCA | GGTAGCAAAATACACCAAAGGTAGG | Start: 47 |
| Tm | 59.082 | 59.875 | End: 209 |
| Size | 21 | 25 | 163 |
