ssr_primer
Primer Details for TRINITY_DN12034_c2_g1_i2 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGTAGGTGGCCGGATTCA | GTCCATGGCCCAACTTCAGA | Start: 1223 |
| Tm | 60.032 | 59.962 | End: 1392 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TAGGTAGGTGGCCGGATTCA | AGTCCATGGCCCAACTTCAG | Start: 1223 |
| Tm | 60.032 | 59.961 | End: 1393 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TGCTCCCAACAATGCCAGAT | TGAATCCGGCCACCTACCTA | Start: 1017 |
| Tm | 59.96 | 60.032 | End: 1242 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ATGCTCCCAACAATGCCAGA | TGAATCCGGCCACCTACCTA | Start: 1016 |
| Tm | 59.96 | 60.032 | End: 1242 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | TAGGTGGCCGGATTCAAACC | GTCCATGGCCCAACTTCAGA | Start: 1227 |
| Tm | 60.035 | 59.962 | End: 1392 |
| Size | 20 | 20 | 166 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGTAGGTGGCCGGATTCA | GTCCATGGCCCAACTTCAGA | Start: 1223 |
| Tm | 60.032 | 59.962 | End: 1392 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TAGGTAGGTGGCCGGATTCA | AGTCCATGGCCCAACTTCAG | Start: 1223 |
| Tm | 60.032 | 59.961 | End: 1393 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TGCTCCCAACAATGCCAGAT | TGAATCCGGCCACCTACCTA | Start: 1017 |
| Tm | 59.96 | 60.032 | End: 1242 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ATGCTCCCAACAATGCCAGA | TGAATCCGGCCACCTACCTA | Start: 1016 |
| Tm | 59.96 | 60.032 | End: 1242 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | TAGGTGGCCGGATTCAAACC | GTCCATGGCCCAACTTCAGA | Start: 1227 |
| Tm | 60.035 | 59.962 | End: 1392 |
| Size | 20 | 20 | 166 |
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