ssr_primer
Primer Details for TRINITY_DN12033_c1_g1_i6 |
|---|
| (CTTC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACCATCAAGATACGCGGA | CCCGGATGATCCTGAAGTGG | Start: 2894 |
| Tm | 59.97 | 59.893 | End: 3076 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | TCGACCATCAAGATACGCGG | CCCGGATGATCCTGAAGTGG | Start: 2893 |
| Tm | 59.97 | 59.893 | End: 3076 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | GCGACGGCGAAGTAATCAAC | ACCTACCGAACCAGGAGTGA | Start: 335 |
| Tm | 59.975 | 59.886 | End: 579 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | TCAGTGAAAAACGGAGCCGA | CGATCTCCCTGAATCCGACG | Start: 3203 |
| Tm | 59.895 | 60.04 | End: 3421 |
| Size | 20 | 20 | 219 |
| Primer 5 details |
|---|
| Sequence | CCTTGGGAAAGGGAACACGA | ATTGCCCACTCCAACTCAGG | Start: 3568 |
| Tm | 59.89 | 59.961 | End: 3714 |
| Size | 20 | 20 | 147 |
(TA)7(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACCATCAAGATACGCGGA | CCCGGATGATCCTGAAGTGG | Start: 2894 |
| Tm | 59.97 | 59.893 | End: 3076 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | TCGACCATCAAGATACGCGG | CCCGGATGATCCTGAAGTGG | Start: 2893 |
| Tm | 59.97 | 59.893 | End: 3076 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | GCGACGGCGAAGTAATCAAC | ACCTACCGAACCAGGAGTGA | Start: 335 |
| Tm | 59.975 | 59.886 | End: 579 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | TCAGTGAAAAACGGAGCCGA | CGATCTCCCTGAATCCGACG | Start: 3203 |
| Tm | 59.895 | 60.04 | End: 3421 |
| Size | 20 | 20 | 219 |
| Primer 5 details |
|---|
| Sequence | CCTTGGGAAAGGGAACACGA | ATTGCCCACTCCAACTCAGG | Start: 3568 |
| Tm | 59.89 | 59.961 | End: 3714 |
| Size | 20 | 20 | 147 |
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