Primer Details for TRINITY_DN12024_c2_g4_i1 | |||
|---|---|---|---|
| (A)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAACCTTCAGTGATGTCTATTCA | TGACACCCCCTTAGCACTCT | Start: 83 |
| Tm | 57.388 | 60.179 | End: 199 |
| Size | 24 | 20 | 117 |
| Primer 2 details | |||
| Sequence | AGAACCTTCAGTGATGTCTATTCA | GTTGACACCCCCTTAGCACT | Start: 83 |
| Tm | 57.388 | 59.599 | End: 201 |
| Size | 24 | 20 | 119 |
| Primer 3 details | |||
| Sequence | AGAACCTTCAGTGATGTCTATTCA | GGTTGACACCCCCTTAGCAC | Start: 83 |
| Tm | 57.388 | 60.608 | End: 202 |
| Size | 24 | 20 | 120 |
| Primer 4 details | |||
| Sequence | AGAACCTTCAGTGATGTCTATTCA | TTGACACCCCCTTAGCACTC | Start: 83 |
| Tm | 57.388 | 59.309 | End: 200 |
| Size | 24 | 20 | 118 |
| Primer 5 details | |||
| Sequence | AGAACCTTCAGTGATGTCTATTCAT | TGACACCCCCTTAGCACTCT | Start: 83 |
| Tm | 57.807 | 60.179 | End: 199 |
| Size | 25 | 20 | 117 |
