ssr_primer
Primer Details for TRINITY_DN11996_c1_g2_i6 |
|---|
| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGACAATACAAGGGGGAA | AGCATCTGATGACGCCCAAA | Start: 895 |
| Tm | 60.035 | 60.035 | End: 1117 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GCACACTGTCTTCAGCCTCT | ATCCGTGGATTTGTGCAGGT | Start: 487 |
| Tm | 59.965 | 59.962 | End: 744 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | ACCTGCACAAATCCACGGAT | TTCCCCCTTGTATTGTCGCC | Start: 725 |
| Tm | 59.962 | 60.035 | End: 914 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | AGCACGGAAAGCTCTTCCTC | TTGCTTTCCCCATTGCATGC | Start: 2627 |
| Tm | 60.037 | 60.036 | End: 2836 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GAGGAAGCACGGAAAGCTCT | TTGCTTTCCCCATTGCATGC | Start: 2622 |
| Tm | 60.037 | 60.036 | End: 2836 |
| Size | 20 | 20 | 215 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGACAATACAAGGGGGAA | AGCATCTGATGACGCCCAAA | Start: 895 |
| Tm | 60.035 | 60.035 | End: 1117 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GCACACTGTCTTCAGCCTCT | ATCCGTGGATTTGTGCAGGT | Start: 487 |
| Tm | 59.965 | 59.962 | End: 744 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | ACCTGCACAAATCCACGGAT | TTCCCCCTTGTATTGTCGCC | Start: 725 |
| Tm | 59.962 | 60.035 | End: 914 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | AGCACGGAAAGCTCTTCCTC | TTGCTTTCCCCATTGCATGC | Start: 2627 |
| Tm | 60.037 | 60.036 | End: 2836 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GAGGAAGCACGGAAAGCTCT | TTGCTTTCCCCATTGCATGC | Start: 2622 |
| Tm | 60.037 | 60.036 | End: 2836 |
| Size | 20 | 20 | 215 |
(AGA)7g(GAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGACAATACAAGGGGGAA | AGCATCTGATGACGCCCAAA | Start: 895 |
| Tm | 60.035 | 60.035 | End: 1117 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GCACACTGTCTTCAGCCTCT | ATCCGTGGATTTGTGCAGGT | Start: 487 |
| Tm | 59.965 | 59.962 | End: 744 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | ACCTGCACAAATCCACGGAT | TTCCCCCTTGTATTGTCGCC | Start: 725 |
| Tm | 59.962 | 60.035 | End: 914 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | AGCACGGAAAGCTCTTCCTC | TTGCTTTCCCCATTGCATGC | Start: 2627 |
| Tm | 60.037 | 60.036 | End: 2836 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GAGGAAGCACGGAAAGCTCT | TTGCTTTCCCCATTGCATGC | Start: 2622 |
| Tm | 60.037 | 60.036 | End: 2836 |
| Size | 20 | 20 | 215 |
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