ssr_primer
Primer Details for TRINITY_DN11993_c0_g1_i9 |
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| (GAAAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAGAGCAGCCTCCAAAGA | AGCCTAGGAAGCATGGAGGA | Start: 1849 |
| Tm | 60.037 | 60.031 | End: 1997 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
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| Sequence | AGAGCAGCCTCCAAAGAACC | AGCCTAGGAAGCATGGAGGA | Start: 1852 |
| Tm | 59.963 | 60.031 | End: 1997 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
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| Sequence | TGAAAGCTCCTCGCCTTCTG | GGATTCGCTTAGGAGGGAGC | Start: 3054 |
| Tm | 60.037 | 59.966 | End: 3159 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
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| Sequence | GGCAAGGGCAAAACGGAAAT | GAGTCGTTACAACTCGGCCA | Start: 467 |
| Tm | 59.965 | 60.039 | End: 689 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
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| Sequence | AGCTTTCGCTTCTGCTTCCT | AGGGACATCGAGGACCTTCA | Start: 2445 |
| Tm | 59.964 | 59.959 | End: 2645 |
| Size | 20 | 20 | 201 |
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