ssr_primer
Primer Details for TRINITY_DN11940_c3_g3_i1 |
|---|
| (CTT)5tttcttcttcttt(TTC)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGACGTTTCCTTCCTCA | ACGACGACGACATTGGTTCA | Start: 1252 |
| Tm | 59.967 | 59.969 | End: 1415 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
|---|
| Sequence | CACCGACGTTTCCTTCCTCA | TTTCGAGGAGGAACACGACG | Start: 1252 |
| Tm | 59.967 | 60.04 | End: 1435 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | ATTCCTCAAGATCACGGCGG | ACGACGACGACATTGGTTCA | Start: 1284 |
| Tm | 60.179 | 59.969 | End: 1415 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | ATTCCTCAAGATCACGGCGG | TTTCGAGGAGGAACACGACG | Start: 1284 |
| Tm | 60.179 | 60.04 | End: 1435 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | CCTCAAGATCACGGCGGTAG | ACGACGACGACATTGGTTCA | Start: 1287 |
| Tm | 60.249 | 59.969 | End: 1415 |
| Size | 20 | 20 | 129 |
(GTC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGACGTTTCCTTCCTCA | ACGACGACGACATTGGTTCA | Start: 1252 |
| Tm | 59.967 | 59.969 | End: 1415 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
|---|
| Sequence | CACCGACGTTTCCTTCCTCA | TTTCGAGGAGGAACACGACG | Start: 1252 |
| Tm | 59.967 | 60.04 | End: 1435 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | ATTCCTCAAGATCACGGCGG | ACGACGACGACATTGGTTCA | Start: 1284 |
| Tm | 60.179 | 59.969 | End: 1415 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | ATTCCTCAAGATCACGGCGG | TTTCGAGGAGGAACACGACG | Start: 1284 |
| Tm | 60.179 | 60.04 | End: 1435 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | CCTCAAGATCACGGCGGTAG | ACGACGACGACATTGGTTCA | Start: 1287 |
| Tm | 60.249 | 59.969 | End: 1415 |
| Size | 20 | 20 | 129 |
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