ssr_primer
Primer Details for TRINITY_DN11940_c3_g2_i17 |
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| (T)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCGCTGTTGCTTCATTTG | TCTACGATGGACATGGGGGT | Start: 3038 |
| Tm | 60.039 | 60.032 | End: 3250 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
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| Sequence | AGCCGCTGTTGCTTCATTTG | ACCACGTGAGTTGGTGTGTT | Start: 3038 |
| Tm | 60.039 | 60.036 | End: 3171 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | CCATCCCCCTGCAGAACTTT | GCCCTGCACCTGACTGTATT | Start: 2521 |
| Tm | 59.96 | 60.035 | End: 2680 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
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| Sequence | AAGACCCAAACGCAAACAGC | GCCCTGCACCTGACTGTATT | Start: 2486 |
| Tm | 59.898 | 60.035 | End: 2680 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
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| Sequence | ACCCCCATGTCCATCGTAGA | ATGCTGCGGAGGATAAAGGG | Start: 3231 |
| Tm | 60.032 | 59.891 | End: 3358 |
| Size | 20 | 20 | 128 |
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