ssr_primer
Primer Details for TRINITY_DN11939_c1_g3_i5 |
|---|
| (GT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAAGGTGGGACTTGGGAG | TTGCGAGGATGATGCGAACT | Start: 157 |
| Tm | 59.962 | 60.108 | End: 305 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | TAGCTTGCCCTCGATCAACC | CTCCCAAGTCCCACCTTTCC | Start: 71 |
| Tm | 59.822 | 59.962 | End: 176 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | TAGCACCCTACAGCCATTGC | TGTGGTGAAGGTGGCGAATT | Start: 1451 |
| Tm | 60.107 | 60.179 | End: 1588 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | TAGCTTGCCCTCGATCAACC | TTGCGAGGATGATGCGAACT | Start: 71 |
| Tm | 59.822 | 60.108 | End: 305 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | TTGCCCTCGATCAACCTGAG | CTCCCAAGTCCCACCTTTCC | Start: 75 |
| Tm | 59.75 | 59.962 | End: 176 |
| Size | 20 | 20 | 102 |
(A)13(AAT)6*: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAAGGTGGGACTTGGGAG | TTGCGAGGATGATGCGAACT | Start: 157 |
| Tm | 59.962 | 60.108 | End: 305 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | TAGCTTGCCCTCGATCAACC | CTCCCAAGTCCCACCTTTCC | Start: 71 |
| Tm | 59.822 | 59.962 | End: 176 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | TAGCACCCTACAGCCATTGC | TGTGGTGAAGGTGGCGAATT | Start: 1451 |
| Tm | 60.107 | 60.179 | End: 1588 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | TAGCTTGCCCTCGATCAACC | TTGCGAGGATGATGCGAACT | Start: 71 |
| Tm | 59.822 | 60.108 | End: 305 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | TTGCCCTCGATCAACCTGAG | CTCCCAAGTCCCACCTTTCC | Start: 75 |
| Tm | 59.75 | 59.962 | End: 176 |
| Size | 20 | 20 | 102 |
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