ssr_primer
Primer Details for TRINITY_DN11919_c3_g2_i5 |
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| (T)22ccttttcttttatataatttttttcccttcctcttaaaaccaacgcaaacccaccaaattttcccc(CT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGTAGCAAGGCCTTGGAA | TCGAAGCCAGTCATTCCAGG | Start: 364 |
| Tm | 59.962 | 59.75 | End: 614 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
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| Sequence | TTCTGGTAGCAAGGCCTTGG | TCGAAGCCAGTCATTCCAGG | Start: 362 |
| Tm | 59.962 | 59.75 | End: 614 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
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| Sequence | TTATGGCTGCCGATTCCCAA | CCAAGGCCTTGCTACCAGAA | Start: 147 |
| Tm | 59.743 | 59.962 | End: 381 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
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| Sequence | TTATGGCTGCCGATTCCCAA | TTCCAAGGCCTTGCTACCAG | Start: 147 |
| Tm | 59.743 | 59.962 | End: 383 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
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| Sequence | TCGCACGATCGGAAACTGAT | CGCACAATCGAACTGGCTTC | Start: 1024 |
| Tm | 59.827 | 60.179 | End: 1125 |
| Size | 20 | 20 | 102 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGTAGCAAGGCCTTGGAA | TCGAAGCCAGTCATTCCAGG | Start: 364 |
| Tm | 59.962 | 59.75 | End: 614 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | TTCTGGTAGCAAGGCCTTGG | TCGAAGCCAGTCATTCCAGG | Start: 362 |
| Tm | 59.962 | 59.75 | End: 614 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TTATGGCTGCCGATTCCCAA | CCAAGGCCTTGCTACCAGAA | Start: 147 |
| Tm | 59.743 | 59.962 | End: 381 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | TTATGGCTGCCGATTCCCAA | TTCCAAGGCCTTGCTACCAG | Start: 147 |
| Tm | 59.743 | 59.962 | End: 383 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | TCGCACGATCGGAAACTGAT | CGCACAATCGAACTGGCTTC | Start: 1024 |
| Tm | 59.827 | 60.179 | End: 1125 |
| Size | 20 | 20 | 102 |
(GAA)6ggtttggaggaggagatg(AGA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGTAGCAAGGCCTTGGAA | TCGAAGCCAGTCATTCCAGG | Start: 364 |
| Tm | 59.962 | 59.75 | End: 614 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | TTCTGGTAGCAAGGCCTTGG | TCGAAGCCAGTCATTCCAGG | Start: 362 |
| Tm | 59.962 | 59.75 | End: 614 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TTATGGCTGCCGATTCCCAA | CCAAGGCCTTGCTACCAGAA | Start: 147 |
| Tm | 59.743 | 59.962 | End: 381 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | TTATGGCTGCCGATTCCCAA | TTCCAAGGCCTTGCTACCAG | Start: 147 |
| Tm | 59.743 | 59.962 | End: 383 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | TCGCACGATCGGAAACTGAT | CGCACAATCGAACTGGCTTC | Start: 1024 |
| Tm | 59.827 | 60.179 | End: 1125 |
| Size | 20 | 20 | 102 |
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