ssr_primer
Primer Details for TRINITY_DN11906_c0_g3_i3 |
|---|
| (TC)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCGGTCTCGAAAACTTCA | TCGCCCGCAATATCATCCTC | Start: 620 |
| Tm | 59.969 | 60.037 | End: 859 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | TCGCGGTCTCGAAAACTTCA | TCGGGTGGTACAGAGCCTTA | Start: 620 |
| Tm | 59.969 | 59.96 | End: 733 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | GGGTTTCGGAAAAACGGAGC | GGTAGAGCGGCAGAATACCC | Start: 2918 |
| Tm | 60.04 | 59.966 | End: 3146 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
|---|
| Sequence | TAAGGCTCTGTACCACCCGA | TCGCCCGCAATATCATCCTC | Start: 714 |
| Tm | 59.96 | 60.037 | End: 859 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | ACAATCCCCCGAAGCAACAT | CTGGGTCCTGGCCTCAAATT | Start: 3894 |
| Tm | 59.96 | 59.96 | End: 4140 |
| Size | 20 | 20 | 247 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCGGTCTCGAAAACTTCA | TCGCCCGCAATATCATCCTC | Start: 620 |
| Tm | 59.969 | 60.037 | End: 859 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | TCGCGGTCTCGAAAACTTCA | TCGGGTGGTACAGAGCCTTA | Start: 620 |
| Tm | 59.969 | 59.96 | End: 733 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | GGGTTTCGGAAAAACGGAGC | GGTAGAGCGGCAGAATACCC | Start: 2918 |
| Tm | 60.04 | 59.966 | End: 3146 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
|---|
| Sequence | TAAGGCTCTGTACCACCCGA | TCGCCCGCAATATCATCCTC | Start: 714 |
| Tm | 59.96 | 60.037 | End: 859 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | ACAATCCCCCGAAGCAACAT | CTGGGTCCTGGCCTCAAATT | Start: 3894 |
| Tm | 59.96 | 59.96 | End: 4140 |
| Size | 20 | 20 | 247 |
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