ssr_primer
Primer Details for TRINITY_DN11878_c1_g1_i1 |
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| (T)10ctaacggaaagtcctttctcatgggtaacccatggctgggaaggttgctttggcgaa(T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTCATGGACCAAAAGCGT | AAAGCAACCTTCCCAGCCAT | Start: 1751 |
| Tm | 59.89 | 60.179 | End: 1937 |
| Size | 20 | 20 | 187 |
| Primer 2 details |
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| Sequence | TGATGTGTGTTGTGGGAGGG | TTGGGCTGCATTGCATTTGG | Start: 1490 |
| Tm | 59.89 | 60.322 | End: 1739 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
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| Sequence | GGAGGGCAAGCGAGAAAGAA | ACGCTTTTGGTCCATGAGGT | Start: 1504 |
| Tm | 60.322 | 59.89 | End: 1770 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
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| Sequence | AGGAGGGCAAGCGAGAAAAA | TTGGGCTGCATTGCATTTGG | Start: 1462 |
| Tm | 59.89 | 60.322 | End: 1739 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
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| Sequence | CCAAATGCAATGCAGCCCAA | AAAGCAACCTTCCCAGCCAT | Start: 1720 |
| Tm | 60.322 | 60.179 | End: 1937 |
| Size | 20 | 20 | 218 |
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