ssr_primer
Primer Details for TRINITY_DN11873_c0_g1_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCGACTTCAAACCCACGA | AAACCCGATTCTCAGCAGCA | Start: 274 |
| Tm | 59.967 | 59.963 | End: 400 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CTCCGACTTCAAACCCACGA | GCCATTGCCAGGTTTCAAGG | Start: 274 |
| Tm | 59.967 | 60.037 | End: 377 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | CTCCGACTTCAAACCCACGA | CGCCATTGAAAGCTGCACTT | Start: 274 |
| Tm | 59.967 | 60.039 | End: 426 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | GAAGCTTGTCGCCATTGCAA | TCGTGGGTTTGAAGTCGGAG | Start: 116 |
| Tm | 60.039 | 59.967 | End: 293 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | CTCCGACTTCAAACCCACGA | ACGAAACCGCCATTGAAAGC | Start: 274 |
| Tm | 59.967 | 60.04 | End: 433 |
| Size | 20 | 20 | 160 |
(AG)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCGACTTCAAACCCACGA | AAACCCGATTCTCAGCAGCA | Start: 274 |
| Tm | 59.967 | 59.963 | End: 400 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CTCCGACTTCAAACCCACGA | GCCATTGCCAGGTTTCAAGG | Start: 274 |
| Tm | 59.967 | 60.037 | End: 377 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | CTCCGACTTCAAACCCACGA | CGCCATTGAAAGCTGCACTT | Start: 274 |
| Tm | 59.967 | 60.039 | End: 426 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | GAAGCTTGTCGCCATTGCAA | TCGTGGGTTTGAAGTCGGAG | Start: 116 |
| Tm | 60.039 | 59.967 | End: 293 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | CTCCGACTTCAAACCCACGA | ACGAAACCGCCATTGAAAGC | Start: 274 |
| Tm | 59.967 | 60.04 | End: 433 |
| Size | 20 | 20 | 160 |
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