ssr_primer
Primer Details for TRINITY_DN11863_c1_g2_i5 |
|---|
| (AT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGAATAGGCAGCAAGA | AGTACCTGGTGCTCACTTGC | Start: 724 |
| Tm | 60.036 | 59.965 | End: 870 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
|---|
| Sequence | CCCACAACAACCTTGCCATG | CTGGAGTTGTTGATGGGGCT | Start: 1980 |
| Tm | 59.966 | 59.961 | End: 2085 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | CGAGTCAGGAGGCACAATGT | TCTTGCTGCCTATTCGCACA | Start: 523 |
| Tm | 60.037 | 60.036 | End: 743 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | AAAAGCGAGCCTTGCGTTTT | TCTTGCTGCCTATTCGCACA | Start: 489 |
| Tm | 59.898 | 60.036 | End: 743 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | TCATGCTGTGCGAATAGGCA | AGTACCTGGTGCTCACTTGC | Start: 718 |
| Tm | 60.108 | 59.965 | End: 870 |
| Size | 20 | 20 | 153 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGAATAGGCAGCAAGA | AGTACCTGGTGCTCACTTGC | Start: 724 |
| Tm | 60.036 | 59.965 | End: 870 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
|---|
| Sequence | CCCACAACAACCTTGCCATG | CTGGAGTTGTTGATGGGGCT | Start: 1980 |
| Tm | 59.966 | 59.961 | End: 2085 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | CGAGTCAGGAGGCACAATGT | TCTTGCTGCCTATTCGCACA | Start: 523 |
| Tm | 60.037 | 60.036 | End: 743 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | AAAAGCGAGCCTTGCGTTTT | TCTTGCTGCCTATTCGCACA | Start: 489 |
| Tm | 59.898 | 60.036 | End: 743 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | TCATGCTGTGCGAATAGGCA | AGTACCTGGTGCTCACTTGC | Start: 718 |
| Tm | 60.108 | 59.965 | End: 870 |
| Size | 20 | 20 | 153 |
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