ssr_primer
Primer Details for TRINITY_DN11860_c0_g3_i1 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCACGAGCTTCAACACCC | GCGTTGGGTTTGAGTGAACC | Start: 978 |
| Tm | 59.966 | 59.97 | End: 1256 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | AGCCATTGGGGAACTCCATG | GTCCACTTTGGGATGAGCGA | Start: 1298 |
| Tm | 60.033 | 60.036 | End: 1565 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | TGATTTGGGTGGAATCCCCG | TACCGGCTTTTCCTTCCCAC | Start: 1858 |
| Tm | 60.034 | 59.963 | End: 2137 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CCAGCAAGTGGTGTTCAAGC | GGTTCCTCCCAAGCATTCCA | Start: 1739 |
| Tm | 59.969 | 59.96 | End: 1918 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGGAATGCTTGGGAGGAACC | TACCTTTGGCCATGGCTCTG | Start: 1899 |
| Tm | 59.96 | 60.034 | End: 2174 |
| Size | 20 | 20 | 276 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCACGAGCTTCAACACCC | GCGTTGGGTTTGAGTGAACC | Start: 978 |
| Tm | 59.966 | 59.97 | End: 1256 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | AGCCATTGGGGAACTCCATG | GTCCACTTTGGGATGAGCGA | Start: 1298 |
| Tm | 60.033 | 60.036 | End: 1565 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | TGATTTGGGTGGAATCCCCG | TACCGGCTTTTCCTTCCCAC | Start: 1858 |
| Tm | 60.034 | 59.963 | End: 2137 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CCAGCAAGTGGTGTTCAAGC | GGTTCCTCCCAAGCATTCCA | Start: 1739 |
| Tm | 59.969 | 59.96 | End: 1918 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGGAATGCTTGGGAGGAACC | TACCTTTGGCCATGGCTCTG | Start: 1899 |
| Tm | 59.96 | 60.034 | End: 2174 |
| Size | 20 | 20 | 276 |
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