Primer Details for TRINITY_DN11847_c5_g4_i2 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGTCAACCTAGCTGAG | TTGAGAAACATAAAGAAGCATAAAGC | Start: 24 |
| Tm | 59.749 | 57.029 | End: 134 |
| Size | 20 | 26 | 111 |
| Primer 2 details |
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| Sequence | AAGGGGGTGTCAACCTAGCT | TTGAGAAACATAAAGAAGCATAAAGC | Start: 21 |
| Tm | 60.476 | 57.029 | End: 134 |
| Size | 20 | 26 | 114 |
| Primer 3 details |
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| Sequence | GGGGTGTCAACCTAGCTGAG | ATTGAGAAACATAAAGAAGCATAAAGC | Start: 24 |
| Tm | 59.749 | 57.422 | End: 135 |
| Size | 20 | 27 | 112 |
| Primer 4 details |
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| Sequence | ACCTAGCTGAGATGTCTGGGT | TTGAGAAACATAAAGAAGCATAAAGC | Start: 33 |
| Tm | 59.992 | 57.029 | End: 134 |
| Size | 21 | 26 | 102 |
| Primer 5 details |
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| Sequence | ATGTCTGGGTGCACCTTCTG | ATGATAAAATCTCAAAGTACAAGAGGT | Start: 44 |
| Tm | 59.963 | 57.034 | End: 168 |
| Size | 20 | 27 | 125 |
