ssr_primers

ssr_primer

Primer Details for TRINITY_DN11836_c2_g1_i7
(AAT)7: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200
(AAT)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200
(AAT)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200
(ATC)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200
(AT)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200
(AT)8(ATA)10*: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200
(A)14: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200
(GA)14: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TACCATGAGCAGCAACAGCA	ATGTCACGTGAGGAGGAGGA	Start: 1240
Tm	59.963	59.96	End: 1377
Size	20	20	138
Primer 2 details
Sequence	AAACGAACCCCAAAACGCTG	TACAAACGCCACCGGAATCA	Start: 2022
Tm	59.898	59.965	End: 2238
Size	20	20	217
Primer 3 details
Sequence	GCTGCGTTTTAGCTCACACC	TACAAACGCCACCGGAATCA	Start: 2038
Tm	60.11	59.965	End: 2238
Size	20	20	201
Primer 4 details
Sequence	GCGTTTTAGCTCACACCGTC	TACAAACGCCACCGGAATCA	Start: 2041
Tm	59.837	59.965	End: 2238
Size	20	20	198
Primer 5 details
Sequence	CTGCGTTTTAGCTCACACCG	TACAAACGCCACCGGAATCA	Start: 2039
Tm	59.836	59.965	End: 2238
Size	20	20	200

CsExSLDb: Transcriptome based Cucumis sativus Extended Shelf Life Database

Primer Details for TRINITY_DN11836_c2_g1_i7

ICAR Data Use Licence