Primer Details for TRINITY_DN11830_c1_g10_i1 | |||
|---|---|---|---|
| (A)13ggctagttcctatttattacatgttctaggtaggttctgttttaattagttcaaaatcgatcttatttccctttttgc(A)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATGTTCTAGGTAGGTTCTGTTT | AGAGAGGTAGCAATGAAGTGTT | Start: 35 |
| Tm | 57.565 | 57.101 | End: 161 |
| Size | 24 | 22 | 127 |
| Primer 2 details | |||
| Sequence | ACATGTTCTAGGTAGGTTCTGTTT | AGTAGAGAGGTAGCAATGAAGTGT | Start: 35 |
| Tm | 57.565 | 58.98 | End: 164 |
| Size | 24 | 24 | 130 |
| Primer 3 details | |||
| Sequence | ACATGTTCTAGGTAGGTTCTGTTT | AGAGAGGTAGCAATGAAGTGTTT | Start: 35 |
| Tm | 57.565 | 57.766 | End: 161 |
| Size | 24 | 23 | 127 |
| Primer 4 details | |||
| Sequence | ACATGTTCTAGGTAGGTTCTGTTTT | AGAGAGGTAGCAATGAAGTGTT | Start: 35 |
| Tm | 58.162 | 57.101 | End: 161 |
| Size | 25 | 22 | 127 |
| Primer 5 details | |||
| Sequence | ACATGTTCTAGGTAGGTTCTGTTT | GTAGAGAGGTAGCAATGAAGTGT | Start: 35 |
| Tm | 57.565 | 57.668 | End: 163 |
| Size | 24 | 23 | 129 |
