ssr_primer
Primer Details for TRINITY_DN11787_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGGGGGCTGAAGCATTAC | GGAGGAGGGATACACTGGGT | Start: 498 |
| Tm | 60.034 | 60.031 | End: 753 |
| Size | 20 | 20 | 256 |
| Primer 2 details |
|---|
| Sequence | TGGGGGCTGAAGCATTACTG | GGAGGAGGGATACACTGGGT | Start: 500 |
| Tm | 60.034 | 60.031 | End: 753 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | ACTGGGGGCTGAAGCATTAC | TGGAGGAGGGATACACTGGG | Start: 498 |
| Tm | 60.034 | 60.031 | End: 754 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | TGGGGGCTGAAGCATTACTG | TGGAGGAGGGATACACTGGG | Start: 500 |
| Tm | 60.034 | 60.031 | End: 754 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | ACCCAGTGTATCCCTCCTCC | ACGGCTTGGACATGACTCTG | Start: 734 |
| Tm | 60.031 | 60.037 | End: 893 |
| Size | 20 | 20 | 160 |
(T)10ctgttttatttaattcttaaatctaagacgagtcaagagaggcaa(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGGGGGCTGAAGCATTAC | GGAGGAGGGATACACTGGGT | Start: 498 |
| Tm | 60.034 | 60.031 | End: 753 |
| Size | 20 | 20 | 256 |
| Primer 2 details |
|---|
| Sequence | TGGGGGCTGAAGCATTACTG | GGAGGAGGGATACACTGGGT | Start: 500 |
| Tm | 60.034 | 60.031 | End: 753 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | ACTGGGGGCTGAAGCATTAC | TGGAGGAGGGATACACTGGG | Start: 498 |
| Tm | 60.034 | 60.031 | End: 754 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | TGGGGGCTGAAGCATTACTG | TGGAGGAGGGATACACTGGG | Start: 500 |
| Tm | 60.034 | 60.031 | End: 754 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | ACCCAGTGTATCCCTCCTCC | ACGGCTTGGACATGACTCTG | Start: 734 |
| Tm | 60.031 | 60.037 | End: 893 |
| Size | 20 | 20 | 160 |
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