ssr_primer
Primer Details for TRINITY_DN11781_c4_g7_i5 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCTTTGCGTGAGATGGGT | GAGGACCAGTGCTCCCTCTA | Start: 2885 |
| Tm | 60.036 | 60.033 | End: 3052 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | TACGCAGTAGAGGGAGCACT | CGATGTCCCAGCTTCCACTT | Start: 3026 |
| Tm | 60.034 | 60.036 | End: 3166 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CTCCTTTGCGTGAGATGGGT | AGTGCTCCCTCTACTGCGTA | Start: 2885 |
| Tm | 60.036 | 60.034 | End: 3045 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | TGGGGATCCACGTTCAAAGG | GAGGACCAGTGCTCCCTCTA | Start: 2928 |
| Tm | 59.962 | 60.033 | End: 3052 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | GGGGATCCACGTTCAAAGGT | GAGGACCAGTGCTCCCTCTA | Start: 2929 |
| Tm | 59.962 | 60.033 | End: 3052 |
| Size | 20 | 20 | 124 |
(A)12gaaaaaaag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCTTTGCGTGAGATGGGT | GAGGACCAGTGCTCCCTCTA | Start: 2885 |
| Tm | 60.036 | 60.033 | End: 3052 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | TACGCAGTAGAGGGAGCACT | CGATGTCCCAGCTTCCACTT | Start: 3026 |
| Tm | 60.034 | 60.036 | End: 3166 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CTCCTTTGCGTGAGATGGGT | AGTGCTCCCTCTACTGCGTA | Start: 2885 |
| Tm | 60.036 | 60.034 | End: 3045 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | TGGGGATCCACGTTCAAAGG | GAGGACCAGTGCTCCCTCTA | Start: 2928 |
| Tm | 59.962 | 60.033 | End: 3052 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | GGGGATCCACGTTCAAAGGT | GAGGACCAGTGCTCCCTCTA | Start: 2929 |
| Tm | 59.962 | 60.033 | End: 3052 |
| Size | 20 | 20 | 124 |
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