ssr_primer
Primer Details for TRINITY_DN11762_c1_g1_i5 |
|---|
| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCAAGCTTGAAGGGTTGC | AGGCCCAGAGGTATGTACGT | Start: 441 |
| Tm | 59.967 | 60.032 | End: 568 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | ACGTACATACCTCTGGGCCT | AACCCAACTCAAATGCCCCA | Start: 549 |
| Tm | 60.032 | 60.105 | End: 758 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | ACCCAACTCAAATGCCCCAT | Start: 549 |
| Tm | 60.032 | 59.883 | End: 757 |
| Size | 20 | 20 | 209 |
| Primer 4 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | GCCTTGCCCTACTGCCTATT | Start: 549 |
| Tm | 60.032 | 59.816 | End: 728 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
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| Sequence | AACTCCACACCATCACCACC | TGGCTATTCTGTGATGGGCC | Start: 1903 |
| Tm | 59.89 | 59.817 | End: 2148 |
| Size | 20 | 20 | 246 |
(AT)7(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCAAGCTTGAAGGGTTGC | AGGCCCAGAGGTATGTACGT | Start: 441 |
| Tm | 59.967 | 60.032 | End: 568 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | AACCCAACTCAAATGCCCCA | Start: 549 |
| Tm | 60.032 | 60.105 | End: 758 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | ACCCAACTCAAATGCCCCAT | Start: 549 |
| Tm | 60.032 | 59.883 | End: 757 |
| Size | 20 | 20 | 209 |
| Primer 4 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | GCCTTGCCCTACTGCCTATT | Start: 549 |
| Tm | 60.032 | 59.816 | End: 728 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | AACTCCACACCATCACCACC | TGGCTATTCTGTGATGGGCC | Start: 1903 |
| Tm | 59.89 | 59.817 | End: 2148 |
| Size | 20 | 20 | 246 |
(TCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCAAGCTTGAAGGGTTGC | AGGCCCAGAGGTATGTACGT | Start: 441 |
| Tm | 59.967 | 60.032 | End: 568 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | AACCCAACTCAAATGCCCCA | Start: 549 |
| Tm | 60.032 | 60.105 | End: 758 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | ACCCAACTCAAATGCCCCAT | Start: 549 |
| Tm | 60.032 | 59.883 | End: 757 |
| Size | 20 | 20 | 209 |
| Primer 4 details |
|---|
| Sequence | ACGTACATACCTCTGGGCCT | GCCTTGCCCTACTGCCTATT | Start: 549 |
| Tm | 60.032 | 59.816 | End: 728 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | AACTCCACACCATCACCACC | TGGCTATTCTGTGATGGGCC | Start: 1903 |
| Tm | 59.89 | 59.817 | End: 2148 |
| Size | 20 | 20 | 246 |
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