ssr_primer
Primer Details for TRINITY_DN11761_c3_g1_i1 |
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| (GAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAGCTCTCAGGCCTACAA | CCCCGTTTGGATCTGAAGCT | Start: 564 |
| Tm | 60.036 | 60.035 | End: 738 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
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| Sequence | GCAAGCTCTCAGGCCTACAA | ATGAACAGCAGGTCAGGAGC | Start: 564 |
| Tm | 60.036 | 60.036 | End: 790 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
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| Sequence | GTGGAAGCAGAAGGTCGACA | TTGGGAGGTTGATGCAGGAC | Start: 136 |
| Tm | 59.966 | 59.962 | End: 255 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
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| Sequence | AGCTTCAGATCCAAACGGGG | ATCGAACACGAGCAAGCAGA | Start: 719 |
| Tm | 60.035 | 60.038 | End: 912 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
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| Sequence | AGCTTCAGATCCAAACGGGG | AGATCGAACACGAGCAAGCA | Start: 719 |
| Tm | 60.035 | 60.038 | End: 914 |
| Size | 20 | 20 | 196 |
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