Primer Details for TRINITY_DN11760_c4_g4_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAGATGGAAAACCTTTTTAGTGT | ACAAAGAAAAGTTTACATCGTAGAACT | Start: 0 |
| Tm | 59.39 | 57.894 | End: 131 |
| Size | 26 | 27 | 132 |
| Primer 2 details |
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| Sequence | GGAAGATGGAAAACCTTTTTAGTGTG | ACAAAGAAAAGTTTACATCGTAGAACT | Start: 1 |
| Tm | 59.076 | 57.894 | End: 131 |
| Size | 26 | 27 | 131 |
| Primer 3 details |
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| Sequence | TGGAAGATGGAAAACCTTTTTAGTG | ACAAAGAAAAGTTTACATCGTAGAACT | Start: 0 |
| Tm | 57.95 | 57.894 | End: 131 |
| Size | 25 | 27 | 132 |
| Primer 4 details |
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| Sequence | GGAAGATGGAAAACCTTTTTAGTGT | ACAAAGAAAAGTTTACATCGTAGAACT | Start: 1 |
| Tm | 57.949 | 57.894 | End: 131 |
| Size | 25 | 27 | 131 |
| Primer 5 details |
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| Sequence | TGGAAGATGGAAAACCTTTTTAGTGT | AGTTTACATCGTAGAACTAAACAGAAA | Start: 0 |
| Tm | 59.39 | 57.246 | End: 122 |
| Size | 26 | 27 | 123 |
