Primer Details for TRINITY_DN11760_c3_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAACCGTTATTTCGCAACTCT | CGATTCCCCTTAAAAACAAATGGGA | Start: 139 |
| Tm | 59.589 | 60.047 | End: 261 |
| Size | 22 | 25 | 123 |
| Primer 2 details | |||
| Sequence | CGAACCGTTATTTCGCAACTCT | TCCGATTCCCCTTAAAAACAAATGG | Start: 139 |
| Tm | 59.589 | 60.047 | End: 263 |
| Size | 22 | 25 | 125 |
| Primer 3 details | |||
| Sequence | CGAACCGTTATTTCGCAACTCT | CGATTCCCCTTAAAAACAAATGGG | Start: 139 |
| Tm | 59.589 | 58.825 | End: 261 |
| Size | 22 | 24 | 123 |
| Primer 4 details | |||
| Sequence | CGAACCGTTATTTCGCAACTCT | CCGATTCCCCTTAAAAACAAATGG | Start: 139 |
| Tm | 59.589 | 58.825 | End: 262 |
| Size | 22 | 24 | 124 |
| Primer 5 details | |||
| Sequence | CGAACCGTTATTTCGCAACTCT | AGATCCGATTCCCCTTAAAAACA | Start: 139 |
| Tm | 59.589 | 57.812 | End: 266 |
| Size | 22 | 23 | 128 |
