ssr_primer
Primer Details for TRINITY_DN11755_c2_g3_i1 |
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| (TTA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAAGCCTGCACAAACGGT | ACAAGAGATGGAAGGGCACG | Start: 1139 |
| Tm | 59.898 | 60.036 | End: 1296 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
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| Sequence | CAGTGTTGTGGCACCATGTG | GACAACTCTTGGCCAATGCG | Start: 529 |
| Tm | 59.969 | 60.11 | End: 770 |
| Size | 20 | 20 | 242 |
| Primer 3 details |
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| Sequence | GAAAAGCCTGCACAAACGGT | GGAAGGGCACGCATGATTTC | Start: 1139 |
| Tm | 59.898 | 59.899 | End: 1287 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | TGCACAAACGGTAGGTGTGA | ACAAGAGATGGAAGGGCACG | Start: 1147 |
| Tm | 59.822 | 60.036 | End: 1296 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | AGTGTTGTGGCACCATGTGA | GACAACTCTTGGCCAATGCG | Start: 530 |
| Tm | 60.107 | 60.11 | End: 770 |
| Size | 20 | 20 | 241 |
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