ssr_primer
Primer Details for TRINITY_DN11750_c1_g7_i4 |
|---|
| (GA)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAACCCTTGCGATCCCTCA | TCACCACGTCATGCGAATCA | Start: 1695 |
| Tm | 60.035 | 60.038 | End: 1927 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | TGGATTCAGAGAGCTGGGGA | TTGCTAGCTCCATTCCTGCC | Start: 1151 |
| Tm | 59.957 | 60.107 | End: 1324 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | CCAAGGTGCTGCGAAGTAGA | ACAAGCACCGTTGATGTGGA | Start: 863 |
| Tm | 60.037 | 60.179 | End: 1010 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | TCCACATCAACGGTGCTTGT | TCCCCAGCTCTCTGAATCCA | Start: 991 |
| Tm | 60.179 | 59.957 | End: 1170 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGTTCCACATCAACGGTGCT | TCCCCAGCTCTCTGAATCCA | Start: 988 |
| Tm | 60.179 | 59.957 | End: 1170 |
| Size | 20 | 20 | 183 |
(AC)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAACCCTTGCGATCCCTCA | TCACCACGTCATGCGAATCA | Start: 1695 |
| Tm | 60.035 | 60.038 | End: 1927 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | TGGATTCAGAGAGCTGGGGA | TTGCTAGCTCCATTCCTGCC | Start: 1151 |
| Tm | 59.957 | 60.107 | End: 1324 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | CCAAGGTGCTGCGAAGTAGA | ACAAGCACCGTTGATGTGGA | Start: 863 |
| Tm | 60.037 | 60.179 | End: 1010 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | TCCACATCAACGGTGCTTGT | TCCCCAGCTCTCTGAATCCA | Start: 991 |
| Tm | 60.179 | 59.957 | End: 1170 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGTTCCACATCAACGGTGCT | TCCCCAGCTCTCTGAATCCA | Start: 988 |
| Tm | 60.179 | 59.957 | End: 1170 |
| Size | 20 | 20 | 183 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAACCCTTGCGATCCCTCA | TCACCACGTCATGCGAATCA | Start: 1695 |
| Tm | 60.035 | 60.038 | End: 1927 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | TGGATTCAGAGAGCTGGGGA | TTGCTAGCTCCATTCCTGCC | Start: 1151 |
| Tm | 59.957 | 60.107 | End: 1324 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | CCAAGGTGCTGCGAAGTAGA | ACAAGCACCGTTGATGTGGA | Start: 863 |
| Tm | 60.037 | 60.179 | End: 1010 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | TCCACATCAACGGTGCTTGT | TCCCCAGCTCTCTGAATCCA | Start: 991 |
| Tm | 60.179 | 59.957 | End: 1170 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | TGTTCCACATCAACGGTGCT | TCCCCAGCTCTCTGAATCCA | Start: 988 |
| Tm | 60.179 | 59.957 | End: 1170 |
| Size | 20 | 20 | 183 |
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